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IVF DNA TESTING SERVICES
What is IVF ?
In vitro fertilization (IVF) is a fertility treatment procedure in which eggs are retrieved from a woman's ovaries and fertilized with sperm outside the body in a laboratory. The fertilized eggs, or embryos, are then transferred into the woman's uterus with the goal of achieving pregnancy.
When is IVF recommended?
IVF may be recommended for couples or individuals experiencing infertility due to various factors, including tubal blockages, ovulatory disorders, endometriosis, male factor infertility, or unexplained infertility. It may also be used in cases where other fertility treatments have been unsuccessful.
Is IVF Common? What is the Success Ratio?
IVF is a complex and emotionally challenging process, but it has helped millions of couples worldwide to achieve their dream of having a baby. The success rates of IVF can vary depending on factors such as the woman's age, the cause of infertility, and the quality of the embryos. It's essential for individuals considering IVF to consult with experienced fertility specialists to discuss their options and develop a personalized treatment plan tailored to their needs.
How is IVF done?
The IVF process typically involves several steps:
- Ovarian Stimulation: The woman undergoes ovarian stimulation using fertility medications to encourage the development of multiple eggs. This is monitored closely with blood tests and ultrasound examinations.
- Egg Retrieval: Once the eggs reach maturity, they are retrieved from the woman's ovaries using a minor surgical procedure called transvaginal ultrasound-guided oocyte retrieval. This procedure is usually performed under sedation or anesthesia.
- Sperm Collection: On the day of egg retrieval, the male partner provides a sperm sample, which is prepared in the laboratory to isolate healthy, motile sperm for fertilization.
- Fertilization: The retrieved eggs are then fertilized with sperm in the laboratory using one of two primary methods: (a) Conventional IVF: Eggs and sperm are combined in a petri dish and left to fertilize naturally. (b) Intracytoplasmic Sperm Injection (ICSI): A single sperm is injected directly into each mature egg to facilitate fertilization, typically used in cases of male factor infertility or previous IVF failures.
- Embryo Culture: After fertilization, the embryos are cultured in the laboratory for several days to allow them to develop and grow.
- Embryo Transfer: Once the embryos reach a certain stage of development (usually at the blastocyst stage, around day 5 or 6 after fertilization), one or more embryos are selected for transfer into the woman's uterus. This procedure is typically performed using a thin catheter inserted through the cervix.
- Embryo Freezing (Cryopreservation): Any remaining viable embryos that are not transferred may be frozen (cryopreserved) for future use, such as in subsequent IVF cycles.
- Pregnancy Test: Approximately two weeks after embryo transfer, a pregnancy test is performed to determine if implantation and pregnancy have occurred.
What is the Role of DNA Testing in IVF?
DNA testing tools are advance process which helps to cover the prominent aspect of IVF testing and offer peace of mind to the parents going in for IVF Treatment. There are different type of DNA tests that gives insight as per the requirement of couples/individuals:
- PGS Testing
- PGD Testing
- Male Infertility Testing
- PARENTAGE Testing
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IVF DNA TESTING : Test Menu / Price List
Male Infertility Testing
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Y chromosome microdeletion testing is typically recommended for men with certain reproductive issues, such as azoospermia (absence of sperm) or severe oligospermia (very low sperm count). The testing helps assess the genetic factors contributing to male infertility. The regions on the Y chromosome that are commonly evaluated in this testing are referred to as AZF (Azoospermia Factor) regions, including AZFa, AZFb, and AZFc.
PGS Testing
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PGS stands for Preimplantation Genetic Screening. It is a specialized genetic test used during in vitro fertilization (IVF) to analyze embryos for chromosomal abnormalities before they are implanted into the uterus. PGS aims to increase the likelihood of a successful pregnancy by selecting embryos with the correct number of chromosomes, thereby reducing the risk of miscarriage and certain genetic conditions.
PGD TESTING
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Preimplantation Genetic Screening (PGS), also known as Preimplantation Genetic Testing for Aneuploidy (PGT-A), is a procedure used during in vitro fertilization (IVF) to screen embryos for chromosomal abnormalities before they are implanted into the uterus. Here's an overview of how PGS testing is typically done: IVF Procedure: The process begins with the collection of eggs from the woman through ovarian stimulation using fertility medications. The eggs are then retrieved and fertilized with sp
PATERNITY TEST-IVF
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Paternity testing after in vitro fertilization (IVF) is conducted to confirm the genetic relationship between the intended father and the child conceived through IVF. While IVF typically involves the use of the genetic material (sperm) of the intended father, there may be circumstances where paternity testing is warranted.
- Sample Collection: The first step in IVF paternity testing involves collecting DNA samples from the alleged father (intended father) and the child. This is usually done using
MATERNITY TEST-IVF
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Maternity testing in the context of in vitro fertilization (IVF) is typically referred to as confirming the genetic relationship between the intended mother and the child conceived through IVF. Here's how maternity testing in IVF works:
- Sample Collection: DNA samples are collected from both the intended mother and the child. This is usually done using a cheek swab or saliva sample.
- Laboratory Analysis: The DNA samples collected from the intended mother and the child are analyzed in a laboratory. T
PRE-NATAL PATERNITY TEST
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A prenatal paternity test, also known as non-invasive prenatal paternity testing (NIPP), is a procedure used to determine the biological relationship between the alleged father and an unborn child during pregnancy. This testing method allows for paternity testing without the need for invasive procedures that pose potential risks to the fetus, such as chorionic villus sampling (CVS) or amniocentesis.
Here's an overview of how prenatal paternity testing typically works:
- Sample Collection: The proced
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WHAT IS PGS TEST
PGS stands for Preimplantation Genetic Screening. It is a specialized genetic test used during in vitro fertilization (IVF) to analyze embryos for chromosomal abnormalities before they are implanted into the uterus. PGS aims to increase the likelihood of a successful pregnancy by selecting embryos with the correct number of chromosomes, thereby reducing the risk of miscarriage and certain genetic conditions.
HOW IS PGS TEST DONE?
Preimplantation Genetic Screening (PGS), also known as Preimplantation Genetic Testing for Aneuploidy (PGT-A), is a procedure used during in vitro fertilization (IVF) to screen embryos for chromosomal abnormalities before they are implanted into the uterus. Here's an overview of how PGS testing is typically done:
- IVF Procedure: The process begins with the collection of eggs from the woman through ovarian stimulation using fertility medications. The eggs are then retrieved and fertilized with sperm in the laboratory to create embryos.
- Embryo Development: Over several days, the fertilized embryos develop in the laboratory under controlled conditions. By around day 5 or 6 after fertilization, the embryos typically reach the blastocyst stage, at which point they consist of hundreds of cells.
- Embryo Biopsy: At the blastocyst stage, a few cells (usually 5-10) are carefully removed from each embryo. This procedure is called embryo biopsy and is typically performed by a skilled embryologist using a specialized microscope and micromanipulation techniques. The biopsy is conducted with minimal disruption to the embryo.
- Genetic Analysis: The cells obtained from the embryo biopsy are then analyzed to determine their chromosomal makeup. This analysis aims to identify any chromosomal abnormalities, such as aneuploidy (the presence of an abnormal number of chromosomes), which can lead to implantation failure, miscarriage, or certain genetic disorders.
- Genetic Screening: The genetic analysis is often performed using techniques such as next-generation sequencing (NGS) or array comparative genomic hybridization (aCGH). These methods allow for the comprehensive screening of all 23 pairs of chromosomes to detect any abnormalities accurately.
- Embryo Selection: Based on the results of the genetic analysis, embryos that are determined to be euploid (having the correct number of chromosomes) are selected for transfer into the woman's uterus during an IVF cycle. This selection process aims to improve the chances of a successful pregnancy by transferring embryos with the highest potential for implantation and healthy development.
Overall, PGS testing aims to improve the success rates of IVF by identifying chromosomally normal embryos for transfer, thereby reducing the risk of implantation failure, miscarriage, and certain genetic conditions. It's important to note that PGS testing is an optional procedure and may not be suitable or necessary for all IVF patients. The decision to undergo PGS testing should be made in consultation with a fertility specialist, considering individual circumstances and preferences.
PGD TEST
PGD testing allows individuals or couples who are carriers of genetic disorders to have greater control over their reproductive choices and helps to minimize the risk of passing on genetic conditions to their children. It's important to consult with a qualified genetic counselor and fertility specialist to determine whether PGD testing is appropriate and to discuss the potential benefits, limitations, and ethical considerations associated with the procedure.
HOW IS PGS & PGD TEST DIFFERENT?
Preimplantation Genetic Screening (PGS) and Preimplantation Genetic Diagnosis (PGD) are different procedures, although they both involve genetic testing of embryos during in vitro fertilization (IVF). Here's a brief comparison of PGS and PGD:
Purpose:
- PGS: Preimplantation Genetic Screening is primarily used to screen embryos for chromosomal abnormalities, such as aneuploidy (abnormal number of chromosomes). PGS aims to identify embryos with the correct number of chromosomes, which are more likely to result in a successful pregnancy and reduce the risk of miscarriage.
- PGD: Preimplantation Genetic Diagnosis is used to test embryos for specific genetic disorders or mutations known to be present in the family. PGD is employed when there is a known risk of transmitting a genetic condition to offspring, allowing for the selection of embryos free from the targeted genetic disorder.
Genetic Analysis:
- PGS: In PGS, embryos are typically screened for chromosomal abnormalities using techniques such as next-generation sequencing (NGS) or array comparative genomic hybridization (aCGH). These methods assess the overall chromosomal makeup of the embryo.
- PGD: In PGD, embryos are tested for specific genetic mutations or disorders associated with a known genetic condition within the family. The genetic analysis focuses on identifying the presence or absence of the targeted genetic mutation.
Selection Criteria:
- PGS: The primary goal of PGS is to select embryos with the correct number of chromosomes (euploid embryos) for transfer, aiming to increase the chances of a successful pregnancy and reduce the risk of miscarriage.
- PGD: The goal of PGD is to identify embryos free from a specific genetic disorder or mutation, allowing for the selection of unaffected embryos for transfer. PGD helps to reduce the risk of passing on the genetic condition to offspring.
MALE INFERTILITY TEST
Y chromosome microdeletion testing is typically recommended for men with certain reproductive issues, such as azoospermia (absence of sperm) or severe oligospermia (very low sperm count). The testing helps assess the genetic factors contributing to male infertility. The regions on the Y chromosome that are commonly evaluated in this testing are referred to as AZF (Azoospermia Factor) regions, including AZFa, AZFb, and AZFc.
PARENTAGE TESTING
Paternity testing after in vitro fertilization (IVF) may be conducted in specific situations where there is a need to confirm the genetic relationship between the intended parents and the child. While IVF typically involves the use of the genetic material (sperm and/or eggs) of the intended parents, there are scenarios in which paternity testing may be warranted:
- Donor Sperm or Egg: In some cases of IVF, donor sperm or eggs may be used if one or both partners have fertility issues or genetic concerns. In such instances, the intended father may choose to undergo paternity testing to confirm his genetic relationship with the child born through IVF.
- Surrogacy: In gestational surrogacy arrangements, where a surrogate carries an embryo created using the genetic material of the intended parents, paternity testing may be requested to confirm the genetic relationship between the intended father and the child born through IVF.
- Legal or Personal Reasons: Some couples or individuals may choose to undergo paternity testing after IVF for personal or legal reasons. This could include situations where there are doubts or disputes regarding parentage, or for peace of mind purposes to confirm biological relationships within the family.
- Medical Necessity: In rare cases, medical circumstances may necessitate paternity testing after IVF. For example, if there are concerns about mislabeling or mix-ups in the IVF laboratory, paternity testing may be conducted to ensure the correct genetic parentage of the child.
Overall, paternity testing after IVF is typically done to provide reassurance, resolve uncertainties, or address legal or medical concerns regarding parentage.